![]() While there are numerous antibody reagents available from various manufacturers, the criteria to select and validate the proper antibody reagents, for flow cytometry analysis, and to interpret the validation results are not well-defined (2). For reproducible flow cytometry analysis, the quality performance of these antibodies is an absolute requirement. The medical director may request additions of new antibodies based on the clinical need to establish a diagnosis and characterize various normal and neoplastic hematolymphoid populations. Antibody reagents are the key components of multiparametric flow cytometry analysis. ![]() The development of multiparametric flow cytometry assays is a complex task requiring a detailed understanding of flow cytometers, selection of markers for immunophenotype, clone selection, fluorochromes and spectral overlap, surface and/or intracellular staining protocols, and data analysis. Multiparametric flow cytometry is one of the leading technologies for cellular analysis because it allows for the simultaneous detection of numerous characteristics of individual cells with relatively high throughput(15). ![]() ![]() Module #21 Selection and Validation Strategy for Adding Antibodies to Flow Cytometry Panelsīy Kalpesh Shah, Amr Rajab, Teri Oldaker, Andrea Illingworth, and Ann Taylor ![]()
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